Journal: Experimental eye research

Article Title: Sympathetic activation leads to Schlemm's canal expansion via increasing vasoactive intestinal polypeptide secretion from trabecular meshwork.

doi: 10.1016/j.exer.2022.109235

Figure Lengend Snippet: Figure 1. Authentication of primary cultured rat trabecular meshwork (TM) cells. (A) Isolation and proliferation of primary TM cells observed under light microscope. a. Tissue scraps cut from corneoscleral rims. Co: cornea; Ir: iris. Blue triangles indicate the location where TM is supposed to reside. b. Primary TM cells started to crawl out of tissue scraps (indi cated by *) and adhere (indicated by black triangles). c. The attached cells gradually formed “clusters” and became contact-inhibited. d-f. Passage 3 TM cells presented typical spindle-like “fibroblast” phenotype and became cobblestone-like once confluent. Scale bars = 100 μm. (B) Immunofluorescence staining of cultured TM cells with anti-fibronectin, anti-laminin, anti-neuron-specific enolase (NSE), anti-factor- Ⅷ-related antigen (FⅧRAg) antibodies. Scale bar = 100 μm. (C–E) Cells were treated with 200 nM dexamethasone (Dex) for 7 days. Immunohistochem istry staining showed that over fifty percent of TM cells were myocilin-positive (the whole cell was yellow-stained) and was statistically different to that of vehicle control. Western blotting with anti- myocilin antibody showed a doublet at 55 kDa for the Dex-treated group. MYOC: myocilin. Scale bar = 300 μm. (F) Cells were treated with 100 nM Dex or ethanol control for 7 days and dyed with phalloidin. Cross-linked actin networks (CLANs) were observed and indicated by white triangles. Scale bars = 100 μm.

Article Snippet: Expression of fibronectin (M00564-3, BOSTER, China, 1:50), laminin (A03522, BOSTER, China, 1:50), neuron-specific enolase D. Xu et al.

Techniques: Cell Culture, Isolation, Light Microscopy, Immunofluorescence, Staining, Control, Western Blot

Journal: iScience

Article Title: Thyroid hormone treatment counteracts cellular phenotypical remodeling in diabetic organs

doi: 10.1016/j.isci.2023.107826

Figure Lengend Snippet: T3 treatment counteracts pathological alterations in both diabetic kidney and heart and in in vitro human models of high glucose injury (A) Representative images from histological PAS staining in lean and ZDF rat kidney sections (left panels) and analysis of glomerulosclerosis index (right panel). (B and C) Representative images (left panels) and quantification (right panel) of glomerular desmin (B) and α-SMA (C) staining in lean and diabetic animals. (D) Representative images (left panels) and quantification (right panels) of Sirius red staining in left ventricular tissue of lean and ZDF rats. (E) Representative images of WT1 and desmin staining in control, glucose-injured, and T3-treated hiPSC-derived kidney organoids. (F) Representative images of fibronectin and troponin staining in control, glucose-injured, and T3-treated hiPSC-derived cardiac organoids. Data are expressed as mean ± SEM, one-way ANOVA corrected with Tukey’s post hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. n = 3–9 rats per group. Scale bars, 50 μm (A), 20 μm (B, F), 25 μm (C), 100 μm (D). Black circles: Lean; black squares: ZDF Veh; black triangles: ZDF T3.

Article Snippet: After washes, organoids were incubated overnight at 4°C with the following primary antibodies: mouse anti-Troponin (Cat#MA5-12960, Thermo Fisher Scientific 1:200), rabbit anti-Connexin43 (Cat#C6219, Sigma-Aldrich, 1:150), rabbit anti-desmin (Cat#ab15200, Abcam, Cambridge, MA, USA, 1:200) and rabbit anti-fibronectin (Cat#AB2040, Merck Life Science, 1:100).

Techniques: In Vitro, Staining, Derivative Assay

Journal: iScience

Article Title: Thyroid hormone treatment counteracts cellular phenotypical remodeling in diabetic organs

doi: 10.1016/j.isci.2023.107826

Figure Lengend Snippet:

Article Snippet: After washes, organoids were incubated overnight at 4°C with the following primary antibodies: mouse anti-Troponin (Cat#MA5-12960, Thermo Fisher Scientific 1:200), rabbit anti-Connexin43 (Cat#C6219, Sigma-Aldrich, 1:150), rabbit anti-desmin (Cat#ab15200, Abcam, Cambridge, MA, USA, 1:200) and rabbit anti-fibronectin (Cat#AB2040, Merck Life Science, 1:100).

Techniques: Recombinant, Plasmid Preparation, Membrane, Enzyme-linked Immunosorbent Assay, DC Protein Assay, Software

Journal: PLoS ONE

Article Title: Suppression of TGF-β pathway by pirfenidone decreases extracellular matrix deposition in ocular fibroblasts in vitro

doi: 10.1371/journal.pone.0172592

Figure Lengend Snippet: Antibodies used in this study.

Article Snippet: rabbit polyclonal fibronectin , DPC Biermann GmbH (Germany) , DP013.

Techniques:

Journal: iScience

Article Title: Downregulation of protein kinase C gamma reduces epithelial property and enhances malignant phenotypes in colorectal cancer cells

doi: 10.1016/j.isci.2022.105501

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal Fibronectin , Merck , Cat#Ab1954, RRID: AB_11213226.

Techniques: Recombinant, Reporter Assay, Expressing, Negative Control, Plasmid Preparation, Software